IBIF Resource List

IBIF hosts five primary imaging instruments, as well as data analysis workstations as outlined below.

This Leica TCS SP8 microscope is well-suited for small animal imaging and electrophysiology experiments.

  • Lasers: 405, 488, 514, 552, 633, and a Mai Tai 680-1040 nm tunable Ti:S
  • Super HyD detectors with Becker and Hickl Time Corrleated single photon counting system
  • Capable of Multiphoton, SHG, spectral imaging, and standard confocal imaging

This spinning-disk confocal can image at speeds up to 1,000 frames per second. 

  • Equipped with Yokogawa spinning disk and Hamamatsu Orca Flash sCMOS camera
  • Lasers: 405, 488, 561, and 640 nm
  • Super-resolution capabilities
  • More than 10 objective options available
  • Ideal for live cell imaging in real time as well as for longer-term time lapse imaging

This through-the-prism TIRF system was built in-house on an Olympus’ inverted microscope body.

  • Lasers: 405, 488, 552, and 647 nm
  • Image capture with Andor iXon EMCCD camera
  • Ideal for imaging of samples on slides that require high speed and/or high sensitivity

This widefield imaging platform with built-in incubation enables multi-day imaging experiments.

  • Imaging options: fluorescence, brightfield, phase contrast
  • Stage inserts allow users to image with microscope slides or well-plates
  • Users have successfully completed 3 – 5 day imaging experiments that were fully-automated

This widefield microscope with sCMOS camera is ideal for basic imaging and sample inspection. Image capture with Hamamatsu Orca Flash Capable of FURA-based imaging experiments.

Software Supported

IBIF supports and encourages users to work with the following software platforms for the purposes of data acquisition and analysis:

  • ImageJ (Fiji version)
    • Source/Vendor: NIH
    • Purpose: Data Analysis
  • LAS X
    • Source/Vendor: Leica
    • Purpose: Data Acquisition and Analysis
  • MetaXpress
    • Source/Vendor: Molecular Devices
    • Purpose: Molecular Devices
  • NIS Elements
    • Source/Vendor: Nikon
    • Purpose: Data Acquisition and Analysis
  • MATLAB
    • Source/Vendor: Mathworks
    • Purpose: Data Analysis
  • Python
    • Source/Vendor: N/A
    • Purpose: Data Analysis
  • R/RStudio/Posit
    • Source/Vendor: N/A
    • Purpose: Data Analysis

Some of these software packages are readily available for individuals to download or access online. All of these packages are available on one or more workstations within the core facility, but users are billed for using the core’s analysis workstations at an hourly rate.

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