Michael G . Nichols, MS, PhD

Department

Physics

Position

Professor

Books

• Auditory and Vestibular Research : Methods and Protocols (Methods in Molecular Biology, Vol. 1427)
  Nichols Michael G., The Single-Molecule Approach to Membrane Protein Stoichiometry [Book Chapter] 2016
• Reviews in Fluorescence 2009
  Anderson Jon P., Metal Enhancement of Near-IR Fluorescence for Molecular Biotechnology Applications [Book Chapter] 2011
• Auditory and Vestibular Research : Methods and Protocols
  Jensen-Smith Heather, Fluorescence microscopy methods in the study of protein structure and function [Book Chapter] 2009

Publications

• Review of scientific instruments
  Aboyewa O. B., Design of a simple radio frequency circuit for implementing the open-ended coaxial probe method for permittivity measurement
  93:11, p. 114705 - 114705 2022
• Biophysical journal
  Nichols Michael G., Impact of cell culture conditions on assessment of cellular metabolism in vitro by NAD(P)H phasor flim with application to in vivo diagnostic imaging for non-invasive biopsy
  121:3, p. 276a - 276a 2022
• Journal of biomedical optics
  Pham Dan L, Development and characterization of phasor-based analysis for FLIM to evaluate the metabolic and epigenetic impact of HER2 inhibition on Squamous Cell Carcinoma (SCC) cultures
  26:10 2021
• Journal of Biomedical Optics
  Pham Dan L., Development and characterization of phasor-based analysis for FLIM to evaluate the metabolic and epigenetic impact of HER2 inhibition on squamous cell carcinoma cultures
  26:10 2021
• ACS Omega
  Bony Badrul Alam, Ultrasmall Mixed Eu-Gd Oxide Nanoparticles for Multimodal Fluorescence and Magnetic Resonance Imaging of Passive Accumulation and Retention in TBI
  5:26, p. 16220 - 16227 2020
• Journal of Drug Targeting
  Meerovich Igor, Low-intensity light-induced drug release from a dual delivery system comprising of a drug loaded liposome and a photosensitive conjugate
  28:6, p. 655 - 667 2020
• Biophysical journal
  Nichols Michael G., Phasor-FLIM Quantification of Changes in Keratinocyte Metabolism and Tissue Architecture in a Longitudinal Study of UV-induced Skin Cancer
  118:3, p. 307a - 307a 2020
• Journal of Drug Targeting
  Meerovich Igor, Low-intensity light-induced paclitaxel release from lipid-based nano-delivery systems
  27:9, p. 971 - 983 2019
• Journal of Biomedical Optics
  Desa Danielle E., Aminoglycosides rapidly inhibit NAD(P)H metabolism increasing reactive oxygen species and cochlear cell demise
  24:5 2019
• Biophysical journal
  Myers Dominick, Two-Photon Imaging of NADH in SKH1 Mice Reveals Changes in Keratinocyte Metabolism with Chronic UVA Exposure
  116:3, p. 281a - 282a 2019
• Biophysical journal
  Nichols Michael G., Metabolic Imaging of Squamous Cell Carcinoma Cells and In Vivo Skin by Phasor FLIM
  112:3, p. 298 - 299a 2017
• Biophysical Journal
  Prathivadhi-Bhayankaram Sruti V., Chemotherapy impedes in vitro microcirculation and promotes migration of leukemic cells with impact on metastasis
  112:3, p. 124a - 124a 2017
• Biophysical journal
  Prathivadhi-Bhayankaram Sruti V., Cell Mechanical Properties and Cancer Metastasis: Effects of Cancer Drugs and Radiotherapy
  110:3, p. 621a - 621a 2016
• Biochemical and Biophysical Research Communications
  Prathivadhi-Bhayankaram Sruti, Chemotherapy Impedes in Vitro Microcirculation and Promotes Migration of Leukemic Cells with Impact on Metastasis
  479:4, p. 841 - 846 2016
• Journal of Biomedical Optics
  Zholudeva L. V., Gentamicin differentially alters cellular metabolism of cochlear hair cells as revealed by NAD(P)H fluorescence lifetime imaging
  20:5, p. (13 pages) 2015
• The FASEB journal
  Cerutis D. Roselyn, Complementing Confocal Detection of Antibody‐labeled Lysophosphatidic Acid Receptors in Human Gingivae with Label‐free Second Harmonic Generation Confocal Microscopy Detection of Collagen
  29:S1 2015
• Biophysical journal
  Desa Danielle, The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells during Ototoxic Aminoglycoside Exposure
  108:2, p. 611a - 611a 2015
• Biophysical journal
  Miller Christina R., Metabolic Profiling of the Skin to Monitor the Onset and Progression of Squamous Cell Carcinoma through Time- and Wavelength-Resolved Fluorescence Lifetime Imaging
  108:2, p. 478 - 478a 2015
• The FASEB journal
  Cerutis D. Roselyn, Label‐free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy (LB41)
  28:S1, p. - n/a 2014
• Biophysical journal
  Smith Heather Jensen, Endogenous Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production during Ototoxin Exposure
  106:2, p. 185a - 185a 2014
• Biophysical journal
  Zholudeva Lyandysha V., Two-Photon Fluorescence Lifetime Imaging for Metabolic Profiling of Cochlear Dysfunction
  106:2, p. 185a - 185a 2014
• Biophysical Journal
  Holman Nathan, One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions
  104:2, p. 346a - 347a 2013
• Microscopy and Microanalysis
  Hallworth Richard J., The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins
  19:4, p. 799 - 807 2013
• Biophysical Journal
  Nichols Michael G., Metabolic Profiling of Multicell Tumor Spheroids by NADH Fluorescence and Spatially-Resolved Oximetry
  104:2, Supplement 1, p. 304a 2013
• Biophysical Journal
  Ward Kristina G., Gentamiacin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging
  104:2, Supplement 1, p. 305a 2013
• Hallworth Richard J., Introduction 2012
• PLoS One
  Jensen-Smith H. C., Gentamicin rapidly inhibits mitochondrial metabolism in high-frequency cochlear outer hair cells.
  7:6, p. e38471 2012
• Journal of Neurophysiology
  Hallworth Richard J., Prestin in HEK cells is an obligate tetramer
  107:1, p. 5 - 11 2012
• Microscopy and Microanalysis
  Vergen Jorge, Metabolic Imaging Using Two-Photon Excited NADH Intensity and Fluorescence Lifetime Imaging
  18:4, p. 761 - 770 2012
• Microscopy and Microanalysis
  Hallworth Richard J., Single Molecule Imaging Approach to Membrane Protein Stoichiometry
  18:4, p. 771 - 780 2012
• Nebraska Academy of Sciences, PO Box 880410 Lincoln NE 68588-0410 United States. [np]. Jun 2011
  Ward Kristina, DEVELOPMENT OF A FREQUENCY-DOMAIN PHASE-SENSITIVE FLUORIMETER TO QUANTIFY THE OXYGEN CONSUMPTION OF LIVING CELLS 2011
• Biophysical journal
  Nichols Michael G., Assessing the Metabolic State of Cultured Cells and the Intact Cochlea by Two-Photon Fluorescence Intensity and Lifetime Imaging
  100:3, p. 319 - 319a 2011
• Biophysical journal
  Thomen Robert P., Single Cell Refractive Index Measurements for Optical Biomechanics
  100:3, p. 141 - 141a 2011
• Biophysical journal
  Tonniges Jeff, Diffusion Measurements of Lipophilic Fluorescent Probes in Fixed Tissue and Living Cells
  98:3, p. 397a - 397a 2010
• Biophysical journal
  Nichols Michael G., A Comparison of Single-Cell Elasticity of Osteogenic Cells Measured with the Optical Stretcher and Holographic Microscope
  98:3, p. 369 - 369a 2010
• Journal of Microscopy
  Tonniges J., Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing
  239:2, p. 117 - 134 2010
• Brain Research
  Tiede LeAnn, Metabolic imaging of the organ of corti — A window on cochlea bioenergetics
  1277, p. 37 - 41 2009
• Nebraska Academy of Sciences, PO Box 880410 Lincoln NE 68588-0410 United States. [np]. Apr 2009
  Smith Timothy, CELL SIGNALLING AND THE OPTICAL STRETCHER 2009
• Nebraska Academy of Sciences, PO Box 880410 Lincoln NE 68588-0410 United States. [np]. Apr 2009
  Vergen Jorge, COMPARISON OF TWO-PHOTON NADH INTENSITY IMAGING AND FLUORESCENCE LIFETIME IMAGING TO QUANTIFY CELLULAR ENERGETICS 2009
• Biophysical journal
  Hansen Maria, Diffusion-Matched and Spectrally-Discrete Lipophilic Probes for Neuronal Tracing
  96:3, p. 151a - 151a 2009
• Biophysical journal
  Tiede LeAnn M., Using Two-photon Excited Fluorescence Intensity- and Lifetime-based NADH Imaging to Investigate Cochlea Metabolism
  96:3, p. 8 - 8a 2009
• Applied Optics
  Ekpenyong Andrew E., Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher
  48:32, p. 6344 - 6354 2009
• Journal of Biomedical Optics
  Tiede Leann M., Determination of hair cell metabolic state in isolated cochlear preparations by two-photon microscopy
  12:2 2007
• Brain Research Bulletin
  Matei V. A., Near-infrared laser illumination transforms the fluorescence absorbing X-Gal reaction product BCI into a transparent, yet brightly fluorescent substance
  70:1, p. 33 - 43 2006
• Brain Research
  Hallworth Richard J., Studying inner ear protein-protein interactions using FRET and FLIM
  1091:1, p. 122 - 131 2006
• Photochemistry and Photobiology
  Tiede Le Ann M., Photobleaching of reduced nicotinamide adenine dinucleotide and the development of highly fluorescent lesions in rat basophilic leukemia cells during multiphoton microscopy
  82:3, p. 656 - 664 2006
• Photochemistry and Photobiology
  Barth Erin E., A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells
  81:3, p. 556 - 562 2005
• Photochemistry and Photobiology
  Barth Erin E., A Comparison of the Sensitivity of Photodamage Assays in Rat Basophilic Leukemia Cells
  81:3, p. 556 - 562 2005
• Photochemistry and Photobiology
  Nichols Michael G., Reduction in DNA synthesis during two-photon microscopy of intrinsic reduced nicotinamide adenine dinucleotide fluorescence
  81:2, p. 259 - 269 2005
• Review of Scientific Instruments
  Bigelow Chad E., Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope
  72:8, p. 3407 - 3410 2001
• Journal of Pharmaceutical Sciences
  Indig Guilherme L., Effect of molecular structure on the performance of triarylmethane dyes as therapeutic agents for photochemical purging of autologous bone marrow grafts from residual tumor cells
  89:1, p. 88 - 99 2000
• Physics in Medicine and Biology
  Hull Edward L., Quantitative broadband near-infrared spectroscopy of tissue-simulating phantoms containing erythrocytes
  43:11, p. 3381 - 3404 1998
• Applied Optics
  Hull Edward L., Localization of luminescent inhomogeneities in turbid media with spatially resolved measurements of cw diffuse luminescence emittance
  37:13, p. 2755 - 2765 1998
• Applied Optics
  Nichols Michael G., Design and testing of a white-light, steady-state diffuse reflectance spectrometer for determination of optical properties of highly scattering systems
  36:1, p. 93 - 104 1997
• Photochemistry and Photobiology
  Georgakoudi Irene, The Mechanism of Photofrin® Photobleaching and Its Consequences for Photodynamic Dosimetry
  65:1, p. 135 - 144 1997
• Physics in Medicine and Biology
  Nichols M. G., Oxygen diffusion and reaction kinetics in the photodynamic therapy of multicell tumour spheroids
  39:12, p. 2161 - 2181 1994
• Cancer Research
  Foster Thomas H., Fluence Rate Effects in Photodynamic Therapy of Multicell Tumor Spheroids
  53:6, p. 1249 - 1254 1993

Presentations

• M.G. Nichols. , K. Brittan, D.L. Che, M. F. Gabriel, M. Glick, B. Huerter, K. Jackson, P. Khoury, D. Myers, P. Nguyen, O. Oladokun, D. L. Pham, K. Sotelo, B. Yang, L. Hansen. In vivo imaging of NAD(P)H by phasor FLIM to monitor changes in metabolism associated with UVinduced skin cancer. NE-INBRE 16th Annual Conference, August 6, 2018, Nebraska City, NE 2018
• M.G. Nichols. The Integrated Biomedical Imaging Core Facility. NE-INBRE 16th Annual Conference, August 6, 2018, Nebraska City, NE 2018
• M. Waid, L. Aumen, J. List, N. Cohorst, M. G. Nichols, A. Csordas, J. Schubert. Operator Exposure in Interventional Radiology. American Association of Physicists in Medicine (AAPM) Annual Meeting, July 29, 2018 Nashville, TN 2018
• K.D. Sotelo, K.A. Jackson, M.F. Gabriel, C.R. Miller, A.M. Mills, O.C. Oladokun, D.L. Pham, B.L. Yang, L.A. Hansen, and M.G. Nichols. Two-photon imaging of NAD(P)H in squamous cell carcinoma to assess metabolic changes due to HER2 using phasor FLIM. National IDEA symposium of Biomedical Research Excellence (NISBRE), June 24- 26, 2018, Washington, D.C. 2018
• M.G. Nichols, K Brittan, M.F. Gabriel, M. Glick, K. Jackson, P. Khoury, P. Nguyen, O. Oladokun, D.L. Pham, K.D. Sotelo, B. Yang, Development of an multiphoton laser-scanning imaging platform for in situ metabolic imaging of disease progression in skin cancer by NAD(P)H FLIM, NE-INBRE 15th Annual Conference, August 6, 2017, Nebraska City, NE 2017
• D.L. Pham, K.D. Sotelo, M.F. Gabriel and Michael G. Nichols, Applying Phasor FLIM to assess the metabolic impact of ultraviolet exposure and HER2 inhibition in cultured Squamous Cell Carcinoma and in vivo skin. 2017 Central Region IDeA Meeting, June 8, 2017. Sioux Falls, SD 2017
• D. R. Cerutis, M.G. Nichols, S.A. Khan, T. Miyamoto, Localization of the Platelet-Activating Factor Receptor on the Intact Human Periodontal Ligament, American Association of Anatomists Annual Meeting at Experimental Biology 2017, April 26, 2017, Chicago, IL 2017
• M.G. Nichols, R. Das, M.F. Gabriel, C.R. Miller, A.M. Mills, D.M. Myers, D.L. Pham, I.N. Pociupany, K. D. Sotelo, K. E. Troup Metabolic imaging of Squamous cell carcinoma cells and in vivo skin by phasor FLIM. Biophysical Society 61st Annual Meeting, February 13, 2017, New Orleans, LA 2017
• M.G. Nichols, M.F. Gabriel, C.R. Miller, A.M. Mills, D.M. Myers, D.L. Pham, I.N. Pociupany, K.D. Sotelo, K.E. Troup, Progress toward metabolic imaging of disease progression in skin cancer by FLIM, NE-INBRE 14th Annual Conference, August 7, 2016, Nebraska City, NE 2016
• D. Desa, E. Riley, M.G. Nichols, H. Jensen Smith. Ototoxic Aminoglycosides inhibit NADH metabolism and increase reactive oxygen species formation in Cochlear cells. Biophysical Society 60th Annual Meeting, March 2, 2016, Los Angeles, CA. 2016
• E. Effa, A. Ikpeme, M. Inyama, B. Agu, T. Ekpenyong, A. Otu, N. Ani,W. Egbe, E.A. Tom, S. Prathivadhi, M.G. Nichols, S. Kosoko-Lasaki, A. Ekpenyong. Mobile Clinics Deployed in Rural Nigeria for Free Patient Care and Clinical Research. Global Health Conference Midwest, February 5, 2016, Omaha, NE. 2016
• C. Taylor , S. Prathivadhi, J. Ning, M.G. Nichols, A. Ekpenyong. Microfluidic devices for biomechanical assessment of metastatic effects of cancer drugs. Global Health Conference Midwest, February 5, 2016, Omaha, NE. 2016
• The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure 2015
• The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure 2015
• Metabolic profiling of the skin to monitor the onset and progression of squamous cell carcinoma through time- and wavelength-resolved fluorescence lifetime imaging 2015
• Shining a light on cellular metabolism of skin cancer through time- and wavelength-resolved multiphoton microscopy 2014
• Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy 2014
• Evaluating mitochondrial superoxide formation in cochlear cells during ototoxic antibiotic exposure 2014
• Metabolic profiling of cochlear dysfunction via two-photon fluorescence lifetime microscopy of NADH 2014
• Mitochondrial respiration studies suggest a novel mechanism for Aminoglycoside-induced hearing loss 2014
• Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy 2014
• Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy 2014
• Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production 2014
• Endogenous Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production during Ototoxin Exposure 2014
• Two-Photon Fluorescence Lifetime Imaging of Cochlear Dysfunction 2014
• Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy 2013
• Assessing the Impact of Antibiotics on Mitochondrial Respiration 2013
• Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared 2013
• Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared 2013
• Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy 2013
• Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy 2013
• The Single-Molecule Approach to Membrane Protein Stoichiometry 2013
• Advanced Methods in Microscopy 2013
• Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy 2013
• Assessing cellular metabolism by NADH FLIM and intensity-based measurements of monolayers and multicell tumor spheroids 2013
• Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids 2013
• Ototoxic antibiotic-induced changes in cochlear metabolism generate cell-damaging free radicals 2013
• Spatially resolved profiling of multicell spheroids by NADH fluorescence lifetime imaging microscopy 2013
• Two-photon fluorescence correlation spectroscopy of proteins in glucose solutions 2013
• Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids 2013
• Biophysical imaging with multiphoton microscopy 2013
• The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins 2013
• Gentamicin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging 2013
• Metabolic profiling of multicell tumor spheroids by NADH fluorescence and spatially-resolved oximetry 2013
• One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions 2013
• Metabolic Imaging using Two-Photon excited NADH Fluorescence Intensity and FLIM 2012
• Fluorescence correlation spectroscopy of proteins in sugar solutions 2012
• Intensity- and lifetime-based analyses of endogenous differences in NADH metabolism in cochlear hair cells 2012
• Two-photon fluorescence intensity and lifetime imaging of NADH reveals glucose availability in cultured cells 2012
• Fluorescence Correlation Spectroscopy of Tryptophan-containing Proteins in Sugar Solutions Using Two Photon Excitation 2012
• Prestin in Membranes Is a Tetramer 2012
• Two-photon fluorescence intensity and lifetime imaging microscopy of cultured cells 2011

Other

• IBIF Core Laboratory Equipment

• The Role of LPA1 in the Etiology of Periodontal Disease

• Safety Assessment of Translational Forces and Torques on Post-Surgical Epicardial Leads During MRI

• IBIF Med Dean Confocal Core Manager Supplemental Funding

• IBIF Core Laboratory Equipment

• The Role of LPA1 in the Etiology of Periodontal Disease

• Safety Assessment of Translational Forces and Torques on Post-Surgical Epicardial Leads During MRI

• IBIF Med Dean Confocal Core Manager Supplemental Funding