IBIF hosts five primary imaging instruments, as well as data analysis workstations as outlined below.

This Leica TCS SP8 microscope is well-suited for small animal imaging and electrophysiology experiments.

  • Lasers: 405, 488, 514, 552, 633, and a Mai Tai 680-1040 nm tunable Ti:S
  • Super HyD detectors with Becker and Hickl Time Corrleated single photon counting system
  • Capable of Multiphoton, SHG, spectral imaging, and standard confocal imaging
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This spinning-disk confocal can image at speeds up to 1,000 frames per second, making it our only confocal microscope that can image in “real time”.

  • Equipped with Yokogawa spinning disk and back-illuminated Photometrics’ Kinetix sCMOS camera for ultrasensitive detection
  • Lasers: 405, 488, 561, and 640 nm
  • Super-resolution capable with Gataca’s Live SR
  • Tokai Hit stage-top incubation system
  • More than 10 objective options available
  • Ideal for live cell imaging in real time as well as for longer-term time lapse imaging
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Nikon Ti2 Spinning Disk Microscope

This through-the-prism TIRF system was built in-house on an Olympus’ inverted microscope body.

  • Lasers: 405, 488, 552, and 647 nm
  • Image capture with Andor iXon EMCCD camera
  • Ideal for imaging of samples on slides that require high speed and/or high sensitivity
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This widefield imaging platform with built-in incubation enables multi-day imaging experiments.

  • Imaging options: fluorescence, brightfield, phase contrast
  • Stage inserts allow users to image with microscope slides or well-plates
  • Users have successfully completed 3 – 5 day imaging experiments that were fully-automated
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This widefield microscope with sCMOS camera is ideal for basic imaging and sample inspection. Image capture with Hamamatsu Orca Flash Capable of FURA-based imaging experiments.

Software Supported

IBIF encourages users to work with vendor-supported software or the FIJI version of ImageJ. In addition, IBIF has a dedicated workstation for the analysis version of Nikon’s NIS Elements, which includes the powerful GA3 analysis platform. If a user has questions regarding analysis software, please ask a member of the team.