Innovative Genomics and Bioinformatics Core (IGBC)

P1

• Read Length: 100bp
• Read Number: 100 million
• Sequencing Cost: $920

P2

• Read Length: 100bp
• Read Number: 500 million
• Sequencing Cost: $1,475

P3

• Read Length: 100bp/200b
• Read Number: 1.2 billion
• Sequencing Cost: $3,151/$4,633

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• Bulk RNA-seq
• Single-cell RNA-seq 
• Whole genome sequencing 
• P1, P2, & P3 reagents available 
• Cost examples using P3 reagents: 
  • $3151 per run (100bp)
    • 5 10x Genomics scRNA-seq samples
      • $630 per sample
    • 60 bulk RNA-seq samples
      • $52 per sample
• Turnaround time: 1 day

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• Library prep for:
  • Single-cell RNA-seq
  • Single-cell ATAC-seq
• Lead time: 2 weeks
• Minimal requirement:
  • Cell/nuclei suspensions 1,000 cells/μL >20μL
• 10,000 target cells per sample
• Cost:
  • $1,878 scRNA-seq

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  RNA quantification, assessment of RNA integrity, DNA quantification, Fragment size analysis 
• Cost:
  • HS 1000 & HS 5000:
    • $5 per lane, $6.50 per ladder
  • HS RNA:
    • $4 per lane, $6.50 per ladder
  • $1 per lane if using non-IGC reagents

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  Quickly quantifies dsDNA, ssDNA, RNA, or protein
• Quantify up to 8 samples at a time
• Cost:
  • $1 per sample

• Cost:
  • $20 per hour 
    • Special price for sequencer users 
  • Turnaround time:
    • 2 days for standard NGS analysis 
    • For customized analyses, time varies
  • Examples:
    • Differential gene expression
    • Copy number alterations
    • Functional annotation of genes 
    • Annotation of SNVs & indels
    • Splicing event detection
    • Etc.

• Cost:
  • Training for standard NGS analysis: $50 an hour
  • Consultations: $20 an hour
  • Special discounts for sequencer users

Director:
Litao Tao, PhD
litaotao@creighton.edu

Core Manager:
Linda Goodman
lindagoodman@creighton.edu

Criss III, Second Floor, Room 259

Sequencer Reservations 
Linda Goodman
lindagoodman@creighton.edu

TapeStation Reservations
Please use the Reservation System to schedule a reservation.

Training/Consultation Reservations
Linda Goodman
lindagoodman@creighton.edu